Powered by Micro-Droplets. Beyond ELISA.

Precision Diagnostics, Multiplied.

What is MELISA Microdroplet Multiplex Diagnostic?

MELISA is a novel immunoassay that prints multiple biomarkers as microdots within a single well, enabling one-time loading and reaction for simultaneous multi-disease or multi-marker detection. It reduces sample and reagent usage, increases throughput, and lowers cost.

  
        Traditional ELISA
        
        MELISA's Multiplex Solution
      
        One well, one target. Scaling requires more wells and plates.
        Throughput
        Multiple targets in a single well.
      
        Large volume due to separate assays
        Sample Usage
         One-time loading for multiple analytes, up to 80 % sample saving.
      
        Requires coating entire wells, high cost of precious antibodies.
        Biomarker Usage
        Just ~10 nL per spot, 99% reduction in biomarker consumption.
      
        Cost grows linearly with test items on reagent and labour.
        Assay Cost
        Cost per test drops as targets increase – shared wells, shared workflows.
      
        Single-channel absorbance reading.
        Output & Analysis
        AI-powered signal recognition, automated reporting.
      
        Moderate. Less sensitive than chemiluminescence or molecular assays.
        Sensitivity
        Can match or surpass CLIA sensitivity through platform upgrade.
      
        Conventional. Equipment and workflow haven't changed in decades.
        Technology
        Self-developed droplet printer, imaging system, and AI analytics.
      
        Disease screening (1 item/test), academic research.
        Applications
        Ideal for syndromic panels, disease subtyping, high-throughput research.

What Makes MELISA Fundamentally Different from Traditional ELISA?

It’s not just about reading more markers — it’s a change in how the foundation of the test is built and how the results are analyzed by recreating both the coating process and the signal development method,

MELISA creates a new paradigm for immunoassays — with higher efficiency, drastically reduced reagent use, and true multiplexing from just a single sample.

Production

Antibodies are coated across the entire well surface, consuming large amounts of reagents with low positional control.

ELISA: "Flood" Coating

Biomarkers are printed as precise nanoliter-sized droplets at fixed positions, minimizing reagent use and ensuring layout control.

MELISA: Precise Microspotting

Detection

Soluble TMB is used and the color diffuses throughout the liquid, allowing for only one visible result per well.

ELISA: Soluble Color Development

A precipitating TMB substrate is used, forming visible colored deposits at each spot, enabling true multiplex detection in a single well.

MELISA: Precipitating TMB

MELISA: Full-Stack Technology Integration

The MELISA team spans biology, electronic engineering, and AI, with end-to-end capabilities covering microdroplet dispensing, diagnostic kit development, and user-end data generation. All core modules are independently developed, forming a fully integrated diagnostic platform.

MELISA Carousel — Refined

Patented pneumatic microdroplet dispenser, adjustable 0.7–20 nL with ~12% CV.
Multiple models: hand-held (research), single-plate (small batch), and mass-production (large scale).
Stable, biocompatible ejection for a wide range of assay applications.

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Microdroplet printing optimization for stable, uniform spots
Surface chemistry tuning for efficient biomarker immobilization
Multiplex layout design to minimize cross-reactivity
Workflow adaptation for reduced reagent use and assay time

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Dengue NS1 Antigen Multiplex – Differentiates all four dengue serotypes; under CDSCO review in India, expected market launch in mid-2026.
Allergy Food Panel Multiplex Screening – Simultaneous detection of ~30 food-related allergens.
Tumor/Cancer Biomarker Multiplex Screening – Concurrent screening of 20+ tumor biomarkers.
Blood Bank Screen – Multiplex detection of Triple-H, syphilis, and other mandatory blood safety markers.

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MI-e100 – High-resolution optical reader for MELISA ELISA microdot assays; captures a full 96-well plate in under 60 seconds.
MI-c105 – Academic-grade cooled CCD imager for high-sensitivity applications.
CMOS-based CLIA Reader (in development) – Next-generation chemiluminescence microdot reader with high dynamic range, compact design, and lower cost for broader adoption.
AI-Integrated Imaging Workflow – One-click operation from image capture to diagnostic reporting with advanced computer vision analysis.

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Automated Spot Recognition & Quantification – AI-driven computer vision for precise signal extraction.
One-Click Workflow – From image acquisition to result reporting in under 60 seconds.
Advanced Data Analytics – Statistical tools for in-depth assay performance and biomarker trend analysis.
Custom System Integration – Adaptable to various laboratory information systems (LIS) and clinical case management systems.

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From New Zealand to the World

The MELISA team is dedicated to delivering precise, practical diagnostics—designed in Aotearoa, sent worldwide

The MELISA Team

MELISA is a novel immunoassay that prints multiple biomarkers as microdots within a single well, enabling one-time loading and reaction for simultaneous multi-disease or multi-marker detection. It reduces sample and reagent usage, increases throughput, and lowers cost.